Influence of trans-ferulic acid on the quality of ram semen upon cold preservation.

ABSTRACT:

Background

Due to lower antioxidant capacity and higher amounts of polyunsaturated fatty acids, ram spermatozoa are very susceptible during cooling process.

Objectives

The objective was to examine the effect of the trans-ferulic acid (t-FA) on the ram semen during liquid preservation.

Methods

Semen samples were collected from the Qezel rams, pooled, and extended with the Tris-based diluent. Pooled samples enriched with different amounts of the t-FA (0, 2.5, 5, 10, and 25 mM) and preserved at 4°C for 72 h. Spermatozoa's kinematics, membrane functionality, and viability were assessed by CASA system, hypoosmotic swelling test, and eosin-nigrosin staining, respectively. Moreover, biochemical parameters were measured at 0, 24, 48, and 72 h.

Results

Results showed th

SUBMITTER: Salimi A 

PROVIDER: S-EPMC10188079 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature