Use of Electron Paramagnetic Resonance (EPR) to Evaluate Redox Status in a Preclinical Model of Acute Lung Injury.

ABSTRACT:

Purpose

Patients with hyper- vs. hypo-inflammatory subphenotypes of acute respiratory distress syndrome (ARDS) exhibit different clinical outcomes. Inflammation increases the production of reactive oxygen species (ROS) and increased ROS contributes to the severity of illness. Our long-term goal is to develop electron paramagnetic resonance (EPR) imaging of lungs in vivo to precisely measure superoxide production in ARDS in real time. As a first step, this requires the development of in vivo EPR methods for quantifying superoxide generation in the lung during injury, and testing if such superoxide measurements can differentiate between susceptible and protected mouse strains.

Procedures

In WT mice, mice lacking total body extracellular superoxide dismutase (EC-SOD) (KO), or m

SUBMITTER: Elajaili HB 

PROVIDER: S-EPMC10188229 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature