Single-cell transcriptomic analysis uncovers diverse and dynamic senescent cell populations.

ABSTRACT: Senescence is a state of enduring growth arrest triggered by sublethal cell damage. Given that senescent cells actively secrete proinflammatory and matrix-remodeling proteins, their accumulation in tissues of older persons has been linked to many diseases of aging. Despite intense interest in identifying robust markers of senescence, the highly heterogeneous and dynamic nature of the senescent phenotype has made this task difficult. Here, we set out to comprehensively analyze the senescent transcriptome of human diploid fibroblasts at the individual-cell scale by performing single-cell RNA-sequencing analysis through two approaches. First, we characterized the different cell states in cultures undergoing senescence triggered by different stresses, and found distinct cell subpopulations tha

SUBMITTER: Wechter N 

PROVIDER: S-EPMC10188353 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature