Impedance spectroscopy of the cell/nanovolcano interface enables optimization for electrophysiology.

ABSTRACT: Volcano-shaped microelectrodes have demonstrated superior performance in measuring attenuated intracellular action potentials from cardiomyocyte cultures. However, their application to neuronal cultures has not yet yielded reliable intracellular access. This common pitfall supports a growing consensus in the field that nanostructures need to be pitched to the cell of interest to enable intracellular access. Accordingly, we present a new methodology that enables us to resolve the cell/probe interface noninvasively through impedance spectroscopy. This method measures changes in the seal resistance of single cells in a scalable manner to predict the quality of electrophysiological recordings. In particular, the impact of chemical functionalization and variation of the probe's geometry can be

SUBMITTER: Maino N 

PROVIDER: S-EPMC10188357 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature