Recording of cellular physiological histories along optically readable self-assembling protein chains.

ABSTRACT: Observing cellular physiological histories is key to understanding normal and disease-related processes. Here we describe expression recording islands-a fully genetically encoded approach that enables both continual digital recording of biological information within cells and subsequent high-throughput readout in fixed cells. The information is stored in growing intracellular protein chains made of self-assembling subunits, human-designed filament-forming proteins bearing different epitope tags that each correspond to a different cellular state or function (for example, gene expression downstream of neural activity or pharmacological exposure), allowing the physiological history to be read out along the ordered subunits of protein chains with conventional optical microscopy. We use express

SUBMITTER: Linghu C 

PROVIDER: S-EPMC10188365 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature