Direct detection and identification of periprosthetic joint infection pathogens by metagenomic next-generation sequencing.

ABSTRACT: This study assessed the application of metagenomic next-generation sequencing in pathogen detection of periprosthetic joint infections. A total of 95 cases who previously had undergone hip and knee replacement undergoing revision from January 2018 to January 2021 were included in this study. Specimens of synovial fluid and deep-tissue were collected for culture and metagenomic next-generation sequencing, and patients were retrospectively categorized as infected or aseptic using the Musculoskeletal Infection Society criteria after revision surgery. The sensitivity, specificity, positive and negative predictive values were compared. A total of 36 cases had positive culture results and 59 cases had positive metagenomic next-generation sequencing results. Culture was positive in 34 infected ca

SUBMITTER: Hao L 

PROVIDER: S-EPMC10188433 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature