A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly.

ABSTRACT: The assembly of the embryo's primary axis is a fundamental landmark for the establishment of the vertebrate body plan. Although the morphogenetic movements directing cell convergence towards the midline have been described extensively, little is known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that the double knockout of yap and its paralog yap1b in medaka results in an axis assembly failure, due to reduced displacement and migratory persistence in mutant cells. Accordingly, we identified genes involved in cytoskeletal organization and cell-ECM adhesion as potentially direct Yap targets. Dynamic analysis of live sensors and downstream targets reveal that Ya

SUBMITTER: Sousa-Ortega A 

PROVIDER: S-EPMC10188487 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature