Effects of HLA single chain trimer design on peptide presentation and stability.

ABSTRACT: MHC class I "single-chain trimer" molecules, coupling MHC heavy chain, β₂-microglobulin, and a specific peptide into a single polypeptide chain, are widely used in research. To more fully understand caveats associated with this design that may affect its use for basic and translational studies, we evaluated a set of engineered single-chain trimers with combinations of stabilizing mutations across eight different classical and non-classical human class I alleles with 44 different peptides, including a novel human/murine chimeric design. While, overall, single-chain trimers accurately recapitulate native molecules, care was needed in selecting designs for studying peptides longer or shorter than 9-mers, as single-chain trimer design could affect peptide conformation. In the

SUBMITTER: Finton KAK 

PROVIDER: S-EPMC10189100 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature