Identification of a novel enhancer essential for Satb1 expression in T_H2 cells and activated ILC2s.

ABSTRACT: The genome organizer, special AT-rich binding protein-1 (SATB1), functions to globally regulate gene networks during primary T cell development and plays a pivotal role in lineage specification in CD4⁺ helper-, CD8⁺ cytotoxic-, and FOXP3⁺ regulatory-T cell subsets. However, it remains unclear how Satb1 gene expression is controlled, particularly in effector T cell function. Here, by using a novel reporter mouse strain expressing SATB1-Venus and genome editing, we have identified a cis-regulatory enhancer, essential for maintaining Satb1 expression specifically in T_H2 cells. This enhancer is occupied by STAT6 and interacts with Satb1 promoters through chromatin looping in T_H2 cells. Reduction of Satb1

SUBMITTER: Nomura A 

PROVIDER: S-EPMC10189277 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature