Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts.

ABSTRACT: Emission fluorescence is one of the most versatile and powerful biophysical techniques used in several scientific subjects. It is extensively applied in the studies of proteins, their conformations, and intermolecular contacts, such as in protein-ligand and protein-protein interactions, allowing qualitative, quantitative, and structural data elucidation. This review, aimed to outline some of the most widely used fluorescence techniques in this area, illustrate their applications and display a few examples. At first, the data on the intrinsic fluorescence of proteins is disclosed, mainly on the tryptophan side chain. Predominantly, research to study protein conformational changes, protein interactions, and changes in intensities and shifts of the fluorescence emission maximums were discusse

SUBMITTER: Dos Santos Rodrigues FH 

PROVIDER: S-EPMC10189374 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature