Transcription activation by the resistance protein AlbA as a tool to evaluate derivatives of the antibiotic albicidin.

ABSTRACT: The rising numbers of fatal infections with resistant pathogens emphasizes the urgent need for new antibiotics. Ideally, new antibiotics should be able to evade or overcome existing resistance mechanisms. The peptide antibiotic albicidin is a highly potent antibacterial compound with a broad activity spectrum but also with several known resistance mechanisms. In order to assess the effectiveness of novel albicidin derivatives in the presence of the binding protein and transcription regulator AlbA, a resistance mechanism against albicidin identified in Klebsiella oxytoca, we designed a transcription reporter assay. In addition, by screening shorter albicidin fragments, as well as various DNA-binders and gyrase poisons, we were able to gain insights into the AlbA target spectrum. We a

SUBMITTER: Kosol S 

PROVIDER: S-EPMC10189885 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature