Cascade autohydrolysis of Alzheimer's Aβ peptides.

ABSTRACT: Protein/peptide self-assembly into amyloid structures associates with major neurodegenerative disorders such as Alzheimer's disease (AD). Soluble assemblies (oligomers) of the Aβ peptide and their aggregates are perceived as neurotoxic species in AD. While screening for synthetic cleavage agents that could break down such aberrant assemblies through hydrolysis, we observed that the assemblies of Aβ oligopeptides, containing the nucleation sequence Aβ_14-24 (H₁₄QKLVFFAEDV₂₄), could act as cleavage agents by themselves. Autohydrolysis showed a common fragment fingerprint among various mutated Aβ_14-24 oligopeptides, Aβ_12-25-Gly and Aβ_1-28, and full-length Aβ_1-40/42, under physiologically relevant conditions. Primary e

SUBMITTER: Wolfram M 

PROVIDER: S-EPMC10189894 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature