Definitive Screening Designs to Optimize Library-Free DIA-MS Identification and Quantification of Neuropeptides.

ABSTRACT: Method optimization is crucial for successful mass spectrometry (MS) analysis. However, extensive method assessments, altering various parameters individually, are rarely performed due to practical limitations regarding time and sample quantity. To maximize sample space for optimization while maintaining reasonable instrumentation requirements, a definitive screening design (DSD) is leveraged for systematic optimization of data-independent acquisition (DIA) parameters to maximize crustacean neuropeptide identifications. While DSDs require several injections, a library-free methodology enables surrogate sample usage for comprehensive optimization of MS parameters to assess biomolecules from limited samples. We identified several parameters contributing significant first- or second-order eff

SUBMITTER: Phetsanthad A 

PROVIDER: S-EPMC10190159 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature