Anionic liposomes prepared without organic solvents for effective siRNA delivery.

ABSTRACT: Currently, organic solvents are necessary for the preparation of anionic liposomes for siRNA delivery. The removal of organic solvent is time-consuming and the residual organic solvent is not only a hidden danger, but also affects the stability of anionic liposomes. Glycerol, which is physiologically compatible and does not need to be removed, is used to promote the dispersion of lipids and the formation of anionic liposomes. Additionally, the preparation process is simple and not time-consuming. The results showed that anionic liposomes, which were typically spherical with a particle size of 188.9 nm were successfully prepared with glycerol. And with the help of Ca²⁺ , siRNA was encapsulated in anionic liposomes. The highest encapsulation efficiency at 2.4 mM Ca²⁺ re

SUBMITTER: Han X 

PROVIDER: S-EPMC10190622 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature