Profiling rhythmicity of bile salt hydrolase activity in the gut lumen with a rapid fluorescence assay.

ABSTRACT: Diurnal rhythmicity of cellular function is key to survival for most organisms on Earth. Many circadian functions are driven by the brain, but regulation of a separate set of peripheral rhythms remains poorly understood. The gut microbiome is a potential candidate for regulation of host peripheral rhythms, and this study sought to specifically examine the process of microbial bile salt biotransformation. To enable this work, an assay for bile salt hydrolase (BSH) that could work with small quantities of stool samples was necessary. Using a turn-on fluorescence probe, we developed a rapid and inexpensive assay to detect BSH enzyme activity with concentrations as low as 6-25 μM, which is considerably more robust than prior approaches. We successfully applied this rhodamine-based assay to det

SUBMITTER: Kombala CJ 

PROVIDER: S-EPMC10191106 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature