Clinical Validity of Tumor-Informed Circulating Tumor DNA Analysis in Patients Undergoing Surgery of Colorectal Metastases.

ABSTRACT:

Background

Accurate biomarkers to monitor tumor load and response in metastatic colorectal cancer patients undergoing surgery could optimize treatment regimens.

Objective

This study explores the clinical validity of tumor-informed quantification of circulating tumor DNA in blood using ultra-deep sequencing.

Design

Resection specimens from 53 colorectal cancer patients were analyzed for tumor-specific mutations in 15 genes. These mutations were used to measure the presence of circulating tumor DNA in preoperatively collected plasma samples using hybrid capture-based sequencing. Additional postoperative measurements were performed one week after surgery in sixteen patients.

Settings

The study was conducted at the Radboud university medical center.

Patients

SUBMITTER: Hofste LSM 

PROVIDER: S-EPMC10191207 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature