Characterization of the l-arabinofuranose-specific GafABCD ABC transporter essential for l-arabinose-dependent growth of the lignocellulose-degrading bacterium <i>Shewanella</i> sp. ANA-3.

ABSTRACT: Microbes that have evolved to live on lignocellulosic biomass face unique challenges in the effective and efficient use of this material as food. The bacterium Shewanella sp. ANA-3 has the potential to utilize arabinan and arabinoxylan, and uptake of the monosaccharide, l-arabinose, derived from these polymers, is known to be mediated by a single ABC transporter. We demonstrate that the substrate binding protein of this system, GafA_Sw, binds specifically to l-arabinofuranose, which is the rare furanose form of l-arabinose found in lignocellulosic biomass. The structure of GafA_Sw was resolved to 1.7 Å and comparison to Escherichia coli YtfQ (GafA_Ec) revealed binding site adaptations that confer specificity for furanose over pyranose forms of mo

SUBMITTER: Drousiotis K 

PROVIDER: S-EPMC10191376 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature