TCN1 Deficiency Inhibits the Malignancy of Colorectal Cancer Cells by Regulating the ITGB4 Pathway.

ABSTRACT:

Background/aims

This study aimed to investigate the biological function and regulatory mechanism of TCN1 in colorectal cancer (CRC).

Methods

We studied the biological function of TCN1 by performing gain-of-function and loss-of-function analyses in HCT116 cell lines; examined the effects of TCN1 on the proliferation, apoptosis, and invasion of CRC cells; and determined potential molecular mechanisms using HCT116 and SW480 CRC lines and mouse xenotransplantation models. Tumor xenograft and colonization assays were performed to detect the tumorigenicity and metastatic foci of cells in vivo.

Results

TCN1 knockdown attenuated CRC cell proliferation and invasion and promoted cell apoptosis. Overexpression of TCN1 yielded the opposite effects. In addition, TCN1-knockd

SUBMITTER: Zhu X 

PROVIDER: S-EPMC10191790 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature