Air-liquid interface cultures trigger a metabolic shift in intestinal epithelial cells (IPEC-1).

ABSTRACT: An improved oxygen availability in air-liquid interface (ALI) cultures of enterocytes of the small intestine seems to be primarily responsible for morphological, metabolic, and functional changes. Intestinal porcine epithelial cells 1 (IPEC-1) are less investigated and are rarely used as model for intestinal barrier but showed a profound change of cell shape during ALI cultivation. We aim to answer the following question: Are the observed morphological effects accompanied by changes in metabolic function? A microarray analysis of submerged culture (SMC) and ALI cultures identified 830 significantly regulated genes. Subsequent functional clustering revealed alterations in 31 pathways, with the highest number of regulated genes in metabolic pathways, carbon metabolism, glycolysis, and hypoxi

SUBMITTER: Stollmeier M 

PROVIDER: S-EPMC10191962 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature