Novel stereological method for estimation of cell counts in 3D collagen scaffolds.

ABSTRACT: Current methods for assessing cell proliferation in 3D scaffolds rely on changes in metabolic activity or total DNA, however, direct quantification of cell number in 3D scaffolds remains a challenge. To address this issue, we developed an unbiased stereology approach that uses systematic-random sampling and thin focal-plane optical sectioning of the scaffolds followed by estimation of total cell number (StereoCount). This approach was validated against an indirect method for measuring the total DNA (DNA content); and the Bürker counting chamber, the current reference method for quantifying cell number. We assessed the total cell number for cell seeding density (cells per unit volume) across four values and compared the methods in terms of accuracy, ease-of-use and time demands. The accurac

SUBMITTER: Zavadakova A 

PROVIDER: S-EPMC10192446 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature