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Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine.

ABSTRACT: The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate network of vessels in the small intestine. For complete details on the use and execution of this protocol, please refer to Karaman et al. (2022).¹.

SUBMITTER: Paavonsalo S

PROVIDER: S-EPMC10192636 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine.

Paavonsalo Satu S Subashi Yelin Y Lackman Madeleine H MH Karaman Sinem S

STAR protocols 20230513 2

The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate netw ...[more]

PMID: 37182207

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