Development of a bioprinter-based method for incorporating metabolic competence into high-throughput in vitro assays.

ABSTRACT: The acceptance and use of in vitro data for hazard identification, prioritization, and risk evaluation is partly limited by uncertainties associated with xenobiotic metabolism. The lack of biotransformation capabilities of many in vitro systems may under- or overestimate the hazard of compounds that are metabolized to more or less active metabolites in vivo. One approach to retrofitting existing bioassays with metabolic competence is the lid-based Alginate Immobilization of Metabolic Enzymes (AIME) method, which adds hepatic metabolism to conventional high-throughput screening platforms. Here, limitations of the lid-based AIME method were addressed by incorporating bioprinting, which involved depositing S9-encapsulated microspheres into standard 384-well plates with re

SUBMITTER: Hopperstad K 

PROVIDER: S-EPMC10192685 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature