Non-hypertrophic chondrogenesis of mesenchymal stem cells through mechano-hypoxia programing.

ABSTRACT: Cartilage tissue engineering aims to generate functional replacements to treat cartilage defects from damage and osteoarthritis. Human bone marrow-derived mesenchymal stem cells (hBM-MSC) are a promising cell source for making cartilage, but current differentiation protocols require the supplementation of growth factors like TGF-β1 or -β3. This can lead to undesirable hypertrophic differentiation of hBM-MSC that progress to bone. We have found previously that exposing engineered human meniscus tissues to physiologically relevant conditions of the knee (mechanical loading and hypoxia; hence, mechano-hypoxia conditioning) increased the gene expression of hyaline cartilage markers, SOX9 and COL2A1, inhibited hypertrophic marker COL10A1, and promoted bulk mechanical proper

SUBMITTER: Li DX 

PROVIDER: S-EPMC10192798 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature