A PCR protocol to establish standards for routine mycoplasma testing that by design detects over ninety percent of all known mycoplasma species.
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ABSTRACT:
SUBMITTER: Siegl D
PROVIDER: S-EPMC10192841 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
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A PCR protocol to establish standards for routine mycoplasma testing that by design detects over ninety percent of all known mycoplasma species.
Siegl Dominik D Kruchem Marie M Jansky Sandrine S Eichler Emma E Thies Dorothe D Hartwig Udo U Schuppan Detlef D Bockamp Ernesto E
iScience 20230426 5
Mycoplasma infection leads to false and non-reproducible scientific data and poses a risk to human health. Despite strict guidelines calling for regular mycoplasma screening, there is no universal and widely established standard procedure. Here, we describe a reliable and cost-effective PCR method that establishes a universal protocol for mycoplasma testing. The applied strategy utilizes ultra-conserved eukaryotic and mycoplasma sequence primers covering by design 92% of all species in the six o ...[more]
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