Monitoring Cell Adhesion on Polycaprolactone-Chitosan Films with Varying Blend Ratios by Quartz Crystal Microbalance with Dissipation.

ABSTRACT: A detailed understanding of the cell adhesion on polymeric surfaces is required to improve the performance of biomaterials. Quartz crystal microbalance with dissipation (QCM-D) as a surface-sensitive technique has the advantage of label-free and real-time monitoring of the cell-polymer interface, providing distinct signal patterns for cell-polymer interactions. In this study, QCM-D was used to monitor human fetal osteoblastic (hFOB) cell adhesion onto polycaprolactone (PCL) and chitosan (CH) homopolymer films as well as their blend films (75:25 and 25:75). Complementary cell culture assays were performed to verify the findings of QCM-D. The thin polymer films were successfully prepared by spin-coating, and relevant properties, i.e., surface morphology, ζ-potential, wettability, film

SUBMITTER: Ozdabak Sert AB 

PROVIDER: S-EPMC10193393 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature