Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma.

ABSTRACT: Lymphocytes are key for immune surveillance of tumors, but our understanding of the spatial organization and physical interactions that facilitate lymphocyte anti-cancer functions is limited. We used multiplexed imaging, quantitative spatial analysis, and machine learning to create high-definition maps of lung tumors from a Kras/Trp53-mutant mouse model and human resections. Networks of interacting lymphocytes ("lymphonets") emerged as a distinctive feature of the anti-cancer immune response. Lymphonets nucleated from small T cell clusters and incorporated B cells with increasing size. CXCR3-mediated trafficking modulated lymphonet size and number, but T cell antigen expression directed intratumoral localization. Lymphonets preferentially harbored TCF1⁺ PD-1⁺ progenit

SUBMITTER: Gaglia G 

PROVIDER: S-EPMC10193529 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature