Improved biosynthesis of heme in Bacillus subtilis through metabolic engineering assisted fed-batch fermentation.

ABSTRACT:

Background

Heme is an iron/porphyrin complex compound, widely used in the health care, food, and pharmaceutical industries. It is more advantageous and attractive to develop microbial cell factories to produce heme by fermentation, with lower production costs and environmentally more friendly procedures than those of the traditional extraction based on animal blood. In this study, Bacillus subtilis, a typical industrial model microorganism of food safety grade, was used for the first time as the host to synthesize heme.

Results

The heme biosynthetic pathway was engineered as four modules, the endogenous C5 pathway, the heterologous C4 pathway, the uroporphyrinogen (urogen) III synthesis pathway, and the downstream synthesis pathway. Knockout of hemX encoding the negative eff

SUBMITTER: Yang S 

PROVIDER: S-EPMC10193802 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature