Digital microfluidics-based digital counting of single-cell copy number variation (dd-scCNV Seq).

ABSTRACT: Single-cell copy number variations (CNVs), major dynamic changes in humans, result in differential levels of gene expression and account for adaptive traits or underlying disease. Single-cell sequencing is needed to reveal these CNVs but has been hindered by single-cell whole-genome amplification (scWGA) bias, leading to inaccurate gene copy number counting. In addition, most of the current scWGA methods are labor intensive, time-consuming, and expensive with limited wide application. Here, we report a unique single-cell whole-genome library preparation approach based on digital microfluidics for digital counting of single-cell Copy Number Variation (dd-scCNV Seq). dd-scCNV Seq directly fragments the original single-cell DNA and uses these f

SUBMITTER: Yu X 

PROVIDER: S-EPMC10193948 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature