Characterisation of pellicle-forming ability in clinical carbapenem-resistant Acinetobacter baumannii.

ABSTRACT:

Background

Acinetobacter baumannii was reported to have resistance towards carbapenems and the ability to form an air-liquid biofilm (pellicle) which contributes to their virulence. The GacSA two-component system has been previously shown to play a role in pellicle formation. Therefore, this study aims to detect the presence of gacA and gacS genes in carbapenem-resistant Acinetobacter baumannii (CRAB) isolates recovered from patients in intensive care units and to investigate their pellicle forming ability.

Methods

The gacS and gacA genes were screened in 96 clinical CRAB isolates using PCR assay. Pellicle formation assay was performed in Mueller Hinton medium and Luria Bertani medium using borosilicate glass tubes and polypropylene

SUBMITTER: Ng HK 

PROVIDER: S-EPMC10194081 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature