Heterosubtypic immune pressure accelerates emergence of influenza A virus escape phenotypes in mice.

ABSTRACT: Rapid antigenic evolution of the influenza A virus surface antigen hemagglutinin undermines protection conferred by seasonal vaccines. Protective correlates targeted by universal vaccines such as cytotoxic T cells or HA stem directed broadly neutralizing antibodies have been shown to select for immune escape mutants during infection. We developed an in vivo serial passage mouse model for viral adaptation and used next generation sequencing to evaluate full genome viral evolution in the context of broadly protective immunity. Heterosubtypic immune pressure increased the incidence of genome-wide single nucleotide variants, though mutations found in early adapted populations were predominantly stochastic in nature. Prolonged adaptation under heterosubtypic immune selection resulted in the man

SUBMITTER: Chu JT 

PROVIDER: S-EPMC10194115 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature