Fluorescently labelled pepino mosaic virus strains share infected tissues and colocalize in cellular aggregates reminiscent of viral replication complexes.

ABSTRACT: Mixed infections of pepino mosaic virus (PepMV) isolates from the EU and CH2 strains are frequent in tomato crops. An asymmetric antagonistic relationship has been described between these strains, making their in planta interaction worthy of study. The aim of this work was to verify if PepMV isolates labelled with fluorescent proteins recapitulate the interactions described for wild type isolates and, if so, to determine the proportion of cells infected with each isolate in single and mixed infected plants. Infectious clones were prepared for PepMV-CH2-GFP and -EU-TagRFP, and also for their reciprocal combination, PepMV-CH2-TagRFP and -EU-GFP, and used to inoculate Nicotiana benthamiana plants. The accumulation of viral RNA followed trends that differed from wild type viruses, with the Pep

SUBMITTER: Alcaide C 

PROVIDER: S-EPMC10194140 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature