Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system.

ABSTRACT: Coxsackievirus A10 (CVA10) is one of enteroviral pathogens that cause the hand, foot, and mouth disease (HFMD). Since CVA10 was reported to be not easily propagated in the Vero cell culture, a feasible manufacture process for producing formalin-inactivated CVA10 vaccine is urgently needed. Several cell lines that commonly used for viral vaccine production was tested for CVA10 (M2014 strain) culture in this study, and our result showed that CVA10 could be easily propagated in the HEK293A cells. A serum-free HEK293A cell culture system was developed for CVA10 production and the yields have reached over 10⁸ TCID₅₀/mL. The biochemical and immunogenic properties of CVA10 particles obtained from this serum-free HEK293A culture were identical to our previous study. Two major

SUBMITTER: Lien SC 

PROVIDER: S-EPMC10194310 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature