Evaluation of reference genes for qRT-PCR studies in the colchicine producing Gloriosa superba L.

ABSTRACT: The flame lily, Gloriosa superba L., is one of the two primary sources of the anti-inflammatory drug, colchicine. Previous studies have shown that a higher level of colchicine production occurs in the rhizomes than in leaves and roots. Earlier precursor feeding and transcriptome analysis of G. superba have provided a putative pathway and candidate genes involved in colchicine biosynthesis. Comparative analysis of expression levels of candidate pathway genes in different tissues of G. superba using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) can reveal highly expressed genes in the rhizome compared to other tissues which could suggest roles of the gene products in colchicine biosynthesis. Normalization is an important step in

SUBMITTER: Johnson N 

PROVIDER: S-EPMC10195008 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature