Confocal microscopy-based estimation of intracellular conductivities in myocardium for modeling of the normal and infarcted heart.

ABSTRACT: Ventricular arrhythmias are the leading cause of mortality in patients with ischemic heart diseases, such as myocardial infarction (MI). Computational simulation of cardiac electrophysiology provides insights into these arrhythmias and their treatment. However, only sparse information is available on crucial model parameters, for instance, the anisotropic intracellular electrical conductivities. Here, we introduced an approach to estimate these conductivities in normal and MI hearts. We processed and analyzed images from confocal microscopy of left ventricular tissue of a rabbit MI model to generate 3D reconstructions. We derived tissue features including the volume fraction of myocytes (V_myo), gap junctions-containing voxels (V_gj), and fibrosis (V_fibrosis)

SUBMITTER: Greiner J 

PROVIDER: S-EPMC10195095 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature