Structural studies of protein-nucleic acid complexes: A brief overview of the selected techniques.

ABSTRACT: Protein-nucleic acid complexes are involved in all vital processes, including replication, transcription, translation, regulation of gene expression and cell metabolism. Knowledge of the biological functions and molecular mechanisms beyond the activity of the macromolecular complexes can be determined from their tertiary structures. Undoubtably, performing structural studies of protein-nucleic acid complexes is challenging, mainly because these types of complexes are often unstable. In addition, their individual components may display extremely different surface charges, causing the complexes to precipitate at higher concentrations used in many structural studies. Due to the variety of protein-nucleic acid complexes and their different biophysical properties, no simple and universal guidel

SUBMITTER: Szpotkowski K 

PROVIDER: S-EPMC10195699 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature