Refined high-content imaging-based phenotypic drug screening in zebrafish xenografts.

ABSTRACT: Zebrafish xenotransplantation models are increasingly applied for phenotypic drug screening to identify small compounds for precision oncology. Larval zebrafish xenografts offer the opportunity to perform drug screens at high-throughput in a complex in vivo environment. However, the full potential of the larval zebrafish xenograft model has not yet been realized and several steps of the drug screening workflow still await automation to increase throughput. Here, we present a robust workflow for drug screening in zebrafish xenografts using high-content imaging. We established embedding methods for high-content imaging of xenografts in 96-well format over consecutive days. In addition, we provide strategies for automated imaging and analysis of zebrafish xenografts including automated tumor

SUBMITTER: Sturtzel C 

PROVIDER: S-EPMC10195872 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature