Activity of a Bacteriophage Cocktail to Control Salmonella Growth Ex Vivo in Avian, Porcine, and Human Epithelial Cell Cultures.

ABSTRACT: We examined the activity of phages to control the growth of chicken and swine Salmonella strains in avian (CHIC-8E11), porcine (IPEC-1), and human (HT-29) cell cultures. We optimized a six-phage cocktail by selecting the five most effective myoviruses and a siphovirus that have optimal lysis on prevalent serovars. We observed ∼20% of 7 log₁₀ PFU/well phage and 3-6 log₁₀ CFU bacterial adhesions, and 3-5 log₁₀ CFU bacterial invasion per 2 cm² of the cultured cells at 2 h post-treatment. The invasive bacteria when plated had a variable reduced susceptibility to the phages. After phage application at an MOI of 10, the prophylaxis regimen had better efficacy at controlling bacterial growth with an up to 6 log₁₀ CFU/well reduction as

SUBMITTER: Nale JY 

PROVIDER: S-EPMC10196083 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature