Evidence for broad cross-reactivity of the SARS-CoV-2 NSP12-directed CD4⁺ T-cell response with pre-primed responses directed against common cold coronaviruses.

ABSTRACT:

Introduction

The nonstructural protein 12 (NSP12) of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) has a high sequence identity with common cold coronaviruses (CCC).

Methods

Here, we comprehensively assessed the breadth and specificity of the NSP12-specific T-cell response after in vitro T-cell expansion with 185 overlapping 15-mer peptides covering the entire SARS-CoV-2 NSP12 at single-peptide resolution in a cohort of 27 coronavirus disease 2019 (COVID-19) patients. Samples of nine uninfected seronegative individuals, as well as five pre-pandemic controls, were also examined to assess potential cross-reactivity with CCCs.

Results

Surprisingly, there was a comparable breadth of individual NSP12 peptide-specific CD4⁺ T-cell re

SUBMITTER: Westphal T 

PROVIDER: S-EPMC10196118 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature