Assembly and analytical validation of a metagenomic reference catalog of human gut microbiota based on co-barcoding sequencing.

ABSTRACT: Human gut microbiota is associated with human health and disease, and is known to have the second-largest genome in the human body. The microbiota genome is important for their functions and metabolites; however, accurate genomic access to the microbiota of the human gut is hindered due to the difficulty of cultivating and the shortcomings of sequencing technology. Therefore, we applied the stLFR library construction method to assemble the microbiota genomes and demonstrated that assembly property outperformed standard metagenome sequencing. Using the assembled genomes as references, SNP, INDEL, and HGT gene analyses were performed. The results demonstrated significant differences in the number of SNPs and INDELs among different individuals. The individual displayed a unique species variat

SUBMITTER: Huang Y 

PROVIDER: S-EPMC10196144 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature