Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse.

ABSTRACT: Protein reabsorption in renal proximal tubules is essential for maintaining nutrient homeostasis. Renal proximal tubule-specific gene knockout is a powerful method to assess the function of genes involved in renal proximal tubule protein reabsorption. However, the lack of inducible renal proximal tubule-specific Cre recombinase-expressing mouse strains hinders the study of gene function in renal proximal tubules. To facilitate the functional study of genes in renal proximal tubules, we developed an AMN ^CreERT2 knock-in mouse strain expressing a Cre recombinase-estrogen receptor fusion protein under the control of the promoter of the amnionless (AMN) gene, a protein reabsorption receptor in renal proximal tubules. AMN ^CreERT2 knock-in

SUBMITTER: Liang S 

PROVIDER: S-EPMC10196630 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature