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Comparison data of transcriptomes from blastocyst seeding samples and cultured cell lines from pigs.

ABSTRACT: Fertilized embryos develop and move freely in the reproductive tract until implantation. Subsequently, the embryos continue to develop after attachment to the uterus. Because of the absence of the uterus, in vitro culturing of embryos is limited to a period of approximately a week. Hatched blastocysts were seeded on feeder cells to extend the culture period. We cultured the colonies formed from the blastocysts for an additional 14 days. From the colonies, four types of cells were established, and each type was isolated to extract RNA. RNA sequencing was conducted using NovaSeq6000. Sequencing reads were aligned to genes and transcripts. Raw data from our previous study were used to compare these samples with the cultured cell lines. We analyzed differentially expressed genes and Gene Ontology terms between new samples and cultured cell lines. Our data can provide essential information for extending the period of embryo culture in vitro.

SUBMITTER: Oh JN

PROVIDER: S-EPMC10196955 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Comparison data of transcriptomes from blastocyst seeding samples and cultured cell lines from pigs.

Oh Jong-Nam JN Jeong Jinsol J Lee Mingyun M Choe Gyung Cheol GC Choi Kwang-Hwan KH Lee Dong-Kyung DK Kim Seung-Hun SH Lee Chang-Kyu CK

Data in brief 20230507

Fertilized embryos develop and move freely in the reproductive tract until implantation. Subsequently, the embryos continue to develop after attachment to the uterus. Because of the absence of the uterus, in vitro culturing of embryos is limited to a period of approximately a week. Hatched blastocysts were seeded on feeder cells to extend the culture period. We cultured the colonies formed from the blastocysts for an additional 14 days. From the colonies, four types of cells were established, an ...[more]

PMID: 37213550

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Dataset Information

Comparison data of transcriptomes from blastocyst seeding samples and cultured cell lines from pigs.

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Comparison data of transcriptomes from blastocyst seeding samples and cultured cell lines from pigs.

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