In Vitro Immunoreactivity Evaluation of H-Ferritin-Based Nanodrugs.

ABSTRACT: Biological nanoparticles, such as proteins and extracellular vesicles, are rapidly growing as nanobased drug-delivery agents due to their biocompatibility, high loading efficiency, and bioavailability. However, most of the candidates emerging preclinically hardly confirm their potential when entering clinical trials. Among other reasons, this is due to the low control of synthesis processes and the limited characterization of their potential immunoreactivity profiles. Here, we propose a combined method that allow us to fully characterize H-ferritin nanoparticles' immunoreactivity during their production, purification, endotoxin removal, and drug loading. H-Ferritin is an extremely interesting nanocage that is being under evaluation for cancer therapy due to its innate cancer tropism, favor

SUBMITTER: Sitia L 

PROVIDER: S-EPMC10197070 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature