A Photoredox Reaction for the Selective Modification of 5-Carboxycytosine in DNA.

ABSTRACT: Covalent epigenetic modifications contribute to the regulation of important cellular processes during development and differentiation, and changes in their genomic distribution and frequency are linked to the emergence of genetic disease states. Chemical and enzymatic methods that selectively target the orthogonal chemical functionality of epigenetic markers are central to the study of their distribution and function, and considerable research effort has been focused on the development of nondestructive sequencing approaches which preserve valuable DNA samples. Photoredox catalysis enables transformations with tunable chemoselectivity under mild, biocompatible reaction conditions. We report the reductive decarboxylation of 5-carboxycytosine via a novel iridium-based treatment, which repres

SUBMITTER: Mortishire-Smith BJ 

PROVIDER: S-EPMC10197125 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature