Liquid-Liquid Phase Separation Modifies the Dynamic Properties of Intrinsically Disordered Proteins.

ABSTRACT: Liquid-liquid phase separation of flexible biomolecules has been identified as a ubiquitous phenomenon underlying the formation of membraneless organelles that harbor a multitude of essential cellular processes. We use nuclear magnetic resonance (NMR) spectroscopy to compare the dynamic properties of an intrinsically disordered protein (measles virus N_TAIL) in the dilute and dense phases at atomic resolution. By measuring ¹⁵N NMR relaxation at different magnetic field strengths, we are able to characterize the dynamics of the protein in dilute and crowded conditions and to compare the amplitude and timescale of the different motional modes to those present in the membraneless organelle. Although the local backbone conformational sampling appears to be largely r

SUBMITTER: Guseva S 

PROVIDER: S-EPMC10197138 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature