Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy.

ABSTRACT:

Significance

Stimulated emission depletion (STED) microscopy has been used to address a wide range of neurobiological questions in optically well-accessible samples, such as cell culture or brain slices. However, the application of STED to deeply embedded structures in the brain of living animals remains technically challenging.

Aim

In previous work, we established chronic STED imaging in the hippocampus in vivo but the gain in spatial resolution was restricted to the lateral plane. In our study, we report on extending the gain in STED resolution into the optical axis to visualize dendritic spines in the hippocampus in vivo.

Approach

Our approach is based on a spatial light modulator to shape the focal STED light intensity in all three dimensions and a c

SUBMITTER: Bancelin S 

PROVIDER: S-EPMC10197143 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature