CircPrime: a web-based platform for design of specific circular RNA primers.

ABSTRACT:

Background

Circular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. Tens of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circRNAs predicted by bioinformatic tools remains an essential part of any circRNA study before publication.

Results

Here, we present the CircPrime web-based platform, providing a user-friendly solution for DNA primer design and thermocycling conditions for circRNA identification with routine PCR methods.

Conclusions

User-friendly CircPrime web platform ( http://circprime.elgene.net/ ) works with outputs of the most popular bioinforma

SUBMITTER: Sharko F 

PROVIDER: S-EPMC10197314 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature