Dataset Information
CircPrime: a web-based platform for design of specific circular RNA primers.
Ontology highlight
ABSTRACT: Background
Circular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. Tens of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circRNAs predicted by bioinformatic tools remains an essential part of any circRNA study before publication.Results
Here, we present the CircPrime web-based platform, providing a user-friendly solution for DNA primer design and thermocycling conditions for circRNA identification with routine PCR methods.Conclusions
User-friendly CircPrime web platform ( http://circprime.elgene.net/ ) works with outputs of the most popular bioinformatic predictors of circRNAs to design specific circular RNA primers. CircPrime works with circRNA coordinates and any reference genome from the National Center for Biotechnology Information database).
SUBMITTER: Sharko F
PROVIDER: S-EPMC10197314 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
Publications
CircPrime: a web-based platform for design of specific circular RNA primers.
BMC bioinformatics 20230519 1
<h4>Background</h4>Circular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. Tens of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circRNAs predicted by bioinformatic tools remains an essential part of any circRNA study before publication.<h4>Results</h4>Here, we present the CircPri ...[more]