Optogenetic inhibition of Gα signalling alters and regulates circuit functionality and early circuit formation.

ABSTRACT: Optogenetic techniques provide genetically targeted, spatially and temporally precise approaches to correlate cellular activities and physiological outcomes. In the nervous system, G-protein-coupled receptors (GPCRs) have essential neuromodulatory functions through binding extracellular ligands to induce intracellular signaling cascades. In this work, we develop and validate a new optogenetic tool that disrupt Gα_q signaling through membrane recruitment of a minimal Regulator of G-protein signaling (RGS) domain. This approach, Photo-induced Modulation of Gα protein - Inhibition of Gα_q (PiGM-Iq), exhibited potent and selective inhibition of Gα_q signaling. We alter the behavior of C. elegans and Drosophila with

SUBMITTER: Lockyer J 

PROVIDER: S-EPMC10197587 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature