Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters.

ABSTRACT: Cellular transcription enables cells to adapt to various stimuli and maintain homeostasis. Transcription factors bind to transcription response elements (TREs) in gene promoters, initiating transcription. Synthetic promoters, derived from natural TREs, can be engineered to control exogenous gene expression using endogenous transcription machinery. This technology has found extensive use in biological research for applications including reporter gene assays, biomarker development, and programming synthetic circuits in living cells. However, a reliable and precise method for selecting minimally-sized synthetic promoters with desired background, amplitude, and stimulation response profiles has been elusive. In this study, we introduce a massively parallel reporter assay library containing 618

SUBMITTER: Zahm AM 

PROVIDER: S-EPMC10197685 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature