Factors influencing the detergent-free membrane protein isolation using synthetic nanodisc-forming polymers.

ABSTRACT: The detergent-free isolation of membrane proteins using synthetic polymers is becoming the desired approach for functional and structural studies of membrane proteins. Since the expression levels for many membrane proteins are low and a high yield of functionalized reconstituted membrane proteins is essential for in vitro studies, it is crucial to optimize the experimental conditions for a given polymer to effectively solubilize target membranes/proteins. The factors that affect membrane solubilization and subsequently the isolation of a target membrane protein include polymer concentration, polymer charge, temperature, pH, and concentration of divalent metal ions. Therefore, it is important to have knowledge about the efficacy of different types of polymers in solubilizing cell mem

SUBMITTER: Krishnarjuna B 

PROVIDER: S-EPMC10197705 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature