Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m⁶A-dependent recognition of Nrf2 mRNA mediated by SND1.

ABSTRACT:

Background

Ferroptosis has been linked to tumor progression and resistance to antineoplastic therapy. Long noncoding RNA (lncRNA) exerts a regulatory role in various biological processes of tumor cells, while the function and molecular mechanism of lncRNA in ferroptosis are yet to be clarified in glioma.

Methods

Both gain-of-function and loss-of-function experiments were employed to investigate the effects of SNAI3-AS1 on the tumorigenesis and ferroptosis susceptibility of glioma in vitro and in vivo. Bioinformatics analysis, Bisulfite sequencing PCR, RNA pull-down, RIP, MeRIP and dual-luciferase reporter assay were performed to explore the low expression mechanism of SNAI3-AS1 and the downstream mechanism of SNAI3-AS1 in ferroptosis susceptibility of glioma.

Results

SUBMITTER: Zheng J 

PROVIDER: S-EPMC10197824 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature